Adapt Yeast for Phytochemical Bioproduction
Phytochemicals are of great pharmaceutical and agricultural significance, yet normally with limiting supplies. Biotechnological production of high-value phytochemicals in the fast growing, fermentable microbes has been considered as an important potential alternative to industrial supply of these expensive molecules. Yeast as one of the most well-studied and utilized eukaryotic microbes, is a favorable host for such bio-production, but with several challenges remain unresolved to date. These challenges are mainly related to the distinct micro-environment in yeast from plant. We are interested in developing a generalized yeast platform for the efficient reconstitution of complex plant pathways with improved bioproduction of target molecules.
Yeast Biosynthesis to Understand Plant Secondary Metabolism
Biosynthesis of most phytochemicals are under investigated, i.e., enzymes catalyzing certain biosynthetic steps are unidentified, and how the pathways are regulated and interact with other plant metabolic pathways are mostly unclear. Using yeast as the model organism, we aim to decipher the biosynthetic pathways of target phytochemicals, characterize enzymatic interactions along the biosynthesis, and map the interactive matrix with other metabolic pathways, This will aid the investigation and understanding of relative plant secondary metabolism, and shed light on developing novel strategies to regulate corresponding biosynthetic pathways.